AOD-9604 Reconstitution Guide
Part of the full AOD-9604 guide - a growth-hormone c-terminal lipolytic fragment analogue reference compound, identity-verified with a COA on every vial.
In brief
This section describes laboratory reconstitution of lyophilized AOD-9604 reference material for in-vitro and preclinical handling. It is a bench-preparation and concentration-math procedure for research material only; it is not a human dosing protocol and contains no personal-use instruction. AOD-9604 is supplied as a lyophilized (freeze-dried) peptide that must be brought into solution before use in laboratory work.
The detail
A closer look
01
Reconstitution principle and concentration math
Lyophilized peptide is dissolved by adding a defined volume of solvent (commonly bacteriostatic water for laboratory handling) to a vial of known peptide mass. The resulting stock concentration equals peptide mass divided by solvent volume. For example, adding 2 mL of bacteriostatic water to a vial containing 5 mg of AOD-9604 yields a stock of 2.5 mg/mL; adding 1 mL to the same 5 mg vial yields 5 mg/mL. Because AOD-9604 has a defined molecular weight of 1815.06 g/mol, a mass concentration can also be expressed in molar terms for assay work using moles = mass / molecular weight. Choosing the solvent volume is purely a function of the working concentration the assay requires.
Step by step
How AOD-9604 is reconstituted
A bench reference for handling and preparation in an in-vitro research setting. Not a personal dosing protocol.
- 1
Bring the lyophilized AOD-9604 vial and the solvent to room temperature before opening to limit condensation on the dry peptide.
- 2
Calculate the solvent volume needed for the target stock concentration using the known vial mass and the relationship concentration = mass / volume.
- 3
Wipe both the peptide vial septum and the bacteriostatic-water vial septum with an alcohol wipe.
- 4
Draw the calculated volume of bacteriostatic water and add it slowly down the inner wall of the peptide vial, letting it run onto the side rather than directly onto the lyophilized pellet.
- 5
Do not shake. Allow the peptide to dissolve undisturbed, with gentle swirling only, until the solution is clear.
- 6
Confirm full dissolution visually; a clear, particle-free solution indicates the reference material is in solution and ready for laboratory aliquoting.
- 7
Label the reconstituted vial with peptide name, stock concentration, and reconstitution date for traceability.
- 8
Store the reconstituted stock refrigerated and protected from light; keep unreconstituted vials frozen and away from light until use.
The fine print: products are sold for laboratory research use only and are not for human or animal consumption. Bodily introduction into humans or animals is strictly prohibited by law. AOD-9604 is not a drug and is not intended to diagnose, treat, cure, or prevent any disease. These statements have not been evaluated by the FDA.