Epitalon Reconstitution Guide
Part of the full Epitalon guide - a synthetic linear tetrapeptide reference compound, identity-verified with a COA on every vial.
In brief
Laboratory handling of Epitalon for in-vitro work centers on reconstituting the supplied lyophilized tetrapeptide into a defined-concentration stock solution suitable for cell-culture and biochemical assays. Because AEDG (Ala-Glu-Asp-Gly) has a low molecular weight of 390.35 g/mol and the molecular formula C14H22N4O9, simple gravimetric-to-molar arithmetic governs how a measured mass dissolves into a chosen diluent volume to reach a target concentration. The principle below describes bench preparation of a research stock using bacteriostatic water as the reconstitution diluent, followed by storage and stability handling for nucleic-acid-binding and telomerase-assay experiments. This is strictly a laboratory preparation workflow for in-vitro research handling and concentration math, not a human dosing protocol. All volumes, concentrations, and storage choices should be confirmed against the certificate of analysis and the requirements of the specific assay, since the entry provides molecular constants but no peptide-specific stability dataset.
The detail
A closer look
01
Reconstitution principle and diluent choice
Reconstitution converts a weighed mass of lyophilized peptide into a liquid stock of known molar concentration. Bacteriostatic water, water containing a low percentage of benzyl alcohol as a preservative, is a common reconstitution diluent for multi-use research vials because the preservative limits microbial growth across repeated withdrawals in a laboratory setting. The diluent is introduced slowly down the inner vial wall rather than directly onto the peptide cake, and the vial is swirled gently rather than shaken, because mechanical agitation and foaming can stress small peptides. For AEDG specifically, the acidic glutamate and aspartate residues make the molecule readily water-soluble, so a clear solution is expected. The molecular weight of 390.35 g/mol is the only constant required to translate a chosen mass and volume into a precise molar concentration for downstream dilution into assay buffer or culture medium.
02
Concentration calculation worked from the molecular weight
Concentration is mass divided by volume, then converted to molarity using the 390.35 g/mol molecular weight. As a worked illustration, dissolving 5 mg of AEDG in 5 mL of bacteriostatic water yields a 1 mg/mL stock; dividing 1 mg/mL by 390.35 g/mol gives approximately 2.56 mM. The same 5 mg in 1 mL would give 5 mg/mL (approximately 12.8 mM), and in 10 mL would give 0.5 mg/mL (approximately 1.28 mM). To hit a defined molar stock directly, multiply the desired molarity by 390.35 to get grams per liter. From a stock of known molarity, serial dilution into assay medium produces the working concentrations used in TRAP, gene-expression, or DNA-binding experiments. Always record the exact mass and volume so the molar concentration delivered to each well is traceable.
Step by step
How Epitalon is reconstituted
A bench reference for handling and preparation in an in-vitro research setting. Not a personal dosing protocol.
- 1
Allow the sealed lyophilized Epitalon vial and the bacteriostatic water to equilibrate to room temperature, and sanitize both stoppers with an alcohol wipe before piercing.
- 2
Decide the target stock concentration and back-calculate the diluent volume from the vial mass using the 390.35 g/mol molecular weight (for example, 5 mg in 5 mL bacteriostatic water gives a 1 mg/mL / approximately 2.56 mM stock).
- 3
Draw the calculated volume of bacteriostatic water into a sterile syringe and add it slowly down the inner wall of the vial so it runs onto, not directly into, the peptide cake.
- 4
Swirl the vial gently until fully dissolved; do not shake or vortex aggressively, and inspect that the solution is clear and particle-free.
- 5
Label the reconstituted vial with peptide name, concentration, diluent, and reconstitution date for traceability.
- 6
Store the reconstituted stock refrigerated for near-term use and aliquot for frozen storage to minimize repeated freeze-thaw cycles; keep the lyophilized starting material per its certificate of analysis.
- 7
Before each assay, serially dilute the stock into the appropriate buffer or culture medium to the defined working concentration, recording the final molarity delivered.
The fine print: products are sold for laboratory research use only and are not for human or animal consumption. Bodily introduction into humans or animals is strictly prohibited by law. Epitalon is not a drug and is not intended to diagnose, treat, cure, or prevent any disease. These statements have not been evaluated by the FDA.