DSIP - Frequently Asked Questions

DSIP is an endogenous amphiphilic neuropeptide, specifically a nonapeptide (nine amino acids), studied in sleep, stress-resistance, and signaling models. It is examined as a multi-pathway modulator rather than as a single-receptor ligand.

The sequence is Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu. The N-terminal tryptophan provides a characteristic UV-absorbance signature used in laboratory identification, and the mix of hydrophilic and hydrophobic residues gives the peptide its amphiphilic character.

DSIP has a molecular weight of 848.81 g/mol, a molecular formula of C35H48N10O15, and CAS number 62568-57-4. These identifiers are used to confirm material identity and to convert between mass and molar concentration in the lab.

In mechanistic work it is reported to interact with components of the MAPK cascade, associated with inhibition of Raf-1 activation and downstream ERK phosphorylation. It also shows homology to the glucocorticoid-induced leucine-zipper (GILZ) protein and is studied for modulation of enkephalin-opioid-receptor interactions.

According to the provided record, DSIP was first isolated in 1977 from rabbit cerebral venous blood. It was originally obtained from cerebral venous blood during induced sleep, which is the historical context that gave the peptide its name.

Three named research areas are MAPK/ERK-cascade signaling models, stress-resistance and neuro-protective assays, and enkephalin-opioid-receptor interaction studies.

No. The reported activities (stress-protective, anti-seizure, immunomodulating) are described as mechanistic, model-system observations and are explicitly not statements of clinical efficacy. The data supports no human-outcome conclusions.

The provided citation is Graf, M. V. and Kastin, A. J., "Delta-sleep-inducing peptide (DSIP): a review," Neuroscience & Biobehavioral Reviews, 1984 (DOI 10.1016/0149-7634(84)90022-8; PMID 6504414).

A lyophilized reference quantity is dissolved in a defined volume of bacteriostatic water to yield a known stock concentration via mass divided by volume. For example, 5 mg in 2 mL gives 2.5 mg/mL. This is a bench-handling procedure for research material, not a human-use protocol.

Divide the mass concentration by the molecular weight (848.81 g/mol). For instance, a 2.5 mg/mL stock corresponds to approximately 2.95 mmol/L (about 2.95 mM). This is an arithmetic illustration of the mass-over-volume principle.

No DSIP-specific half-life, clearance, or degradation rate is provided in the entry. Any stability discussion must rely on general short-peptide principles (hydrolysis sensitivity in solution, peptidase cleavage in biological matrices) and should not be quoted as a DSIP-specific number.

Because no DSIP-specific shelf-life is provided, general peptide-class practice applies: keep the lyophilized solid cold and dry, refrigerate the reconstituted working solution, minimize warming, and aliquot for longer-term frozen storage to limit freeze-thaw cycles. The tryptophan UV signature allows non-destructive spectrophotometric monitoring over time.